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| Registros recuperados: 140 | |
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| Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andrew; King, J. W.; Turner, J. R.; Lapegue, Sylvie. |
| The Pacific oyster, Crassostrea gigas, native to northeast Asia, has been translocated widely for the purpose of aquaculture and is among the most important cultured shellfish. C. gigas has a complex history of introductions in Europe. In France, massive introduction was performed in the early 1970’s, allowing massive spatfall in favourable areas to become the basis of the local oyster industry. In the U.K, hatchery propagation was used to supply local production. Some seed was also exported to Northern Europe were demographically independent populations have been reported in the last decades. In the present study 22 sites were sampled in the British Isles, Denmark, France and Spain in order to assess the genetic diversity and population differentiation... |
| Tipo: Text |
Palavras-chave: Invasion genetics; Pacific oyster; Crassostrea gigas; British Isles. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00167/27814/26007.pdf |
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| Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andy; King, Jonathan W.; Turner, John R.; Lapegue, Sylvie. |
| The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and... |
| Tipo: Text |
Palavras-chave: Invasion genetics; Marine mollusc; Microsatellites; MtDNA; Pacific oyster; Crassostrea gigas. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00268/37973/36052.pdf |
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| Pouvreau, Stephane; Cochet, Hélène; Fabien, Aurélie; Arzul, Isabelle; Lapegue, Sylvie; Gachelin, Sonia; Salaun, Benoit. |
| L’huitre plate, la seule huitre native des côtes européennes, est une espèce emblématique de Bretagne. Convoitée depuis l'Antiquité, sur-pêchée au 19ème siècle, fleuron de l'ostréiculture au 20ème, elle est désormais menacée de disparition à l'aube du 21ème. Sous l'impulsion de la commission OSPAR mais aussi dans un contexte grandissant de crise écologique et d'érosion de la biodiversité imposant une nécessaire conservation et/ou restauration de nos patrimoines naturels, une alliance européenne réunissant biologistes et gestionnaires a été créée en novembre 2017 à Berlin sous l'égide de l'Agence Allemande de Conservation de la Nature (BFN) avec un objectif commun: favoriser le retour de l'huitre plate sur les côtes européennes. Cette alliance pour la... |
| Tipo: Text |
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| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00683/79506/82154.pdf |
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| Batista, Frederico; Boudry, Pierre; Lapegue, Sylvie; Heurtebise, Serge; Monteiro, C. C.. |
| The Portuguese oyster, Crassostrea angulata, was introduced from Portugal to the French Atlantic Coast in the 1860s. C. angulata quickly settled and expanded and leaded to the development of a new aquacultural industry in France. In the late 1960s, mortality associated with the detection of an iridovirus, led to the wipe out of C. angulata from French Atlantic waters and to the massive introduction of C. gigas to sustain production. In Southern Europe, similar symptoms were also observed in natural stocks of C. angulata from Sado River (Portugal) and from the area of Cadiz (Spain). Nowadays, only very few «pure» populations of C. angulata remain in southern Europe. These populations are potentially endangered by the current expansion of C. gigas... |
| Tipo: Text |
Palavras-chave: Selection; Hybridization; Settlement pattern; Population genetic; Genetic; Oysters; Crassostrea angulata; Crassostrea gigas. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3310.pdf |
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| Lapegue, Sylvie; Renault, Tristan. |
| The most known and certainly the most important contribution of genetics to the oyster indus try is the development of the triploid cupped oyster. Released for the first time in the 1980s, the triploid oyster has triplets of chromosomes instead of pairs. Currently, triploid oysters are obtained by crossing diploid and tetraploid oysters. These oysters are characterized by a near lack of milt in summer, which makes them generally popular with consumers, and by a faster growth, allowing a shorter production cycle. In parallel, the exploitation of natural resistance in the diversity of French oysters has been considered to improve the health of farmed populations which is a major challenge for the sustainability of aquaculture farms. Currently, the... |
| Tipo: Text |
Palavras-chave: Huître creuse; Triploïdie; Sélection; Maladies; Ostréiculture; Cupped oyster; Triploidy; Selection; Disease; Shellfish farming. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00498/60931/64326.pdf |
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| Caill-milly, Nathalie; Morandeau, Gilles; Castege, Iker; Sanchez, Florence; Lissardy, Muriel; Chust, Guillem; Borja, Angel; Auby, Isabelle; Lapegue, Sylvie; Prou, Jean; Oger-jeanneret, Helene; Lassalle, Géraldine; Prevost, Etienne; Buoro, Mathieu; Lobry, Jérémy; De Pontual, Helene; De Casamajor, Marie-noelle; Biais, Gerard; D'Elbee, Jean. |
| L'exploitation des ressources par la pêche professionnelle et par la conchyliculture repose sur des ressources vivantes sauvages ou domestiquées qui sont présentes dans différents milieux aquatiques depuis les rivières jusqu'au large en mer. L'état de ces populations dépend non seulement des choix d'exploitation au regard des capacités de renouvellement des stocks mais aussi des caractéristiques environnementales qui impactent les différentes phases des cycles biologiques des espèces et jouent donc sur leur bon déroulement. Ces conditions du milieu sont tributaires pour partie des activités anthropiques (qualité et quantité d'eau disponible, obstacles au franchissement, autres perturbations) et peuvent être soumises aux effets du changement climatique. Ce... |
| Tipo: Text |
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| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00444/55568/57180.pdf |
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| Sauvage, Christopher; Heurtebise, Serge; De Koning, D.; Haley, Chris; Boudry, Pierre; Lapegue, Sylvie. |
| Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). Such selective breeding would strongly benefit from the identification of markers associated to the resistance to allow the development of markers assisted selection. Recently, a set of a new kinds of markers (SNPs) have been developed in the Pacific oyster. These markers were identified and characterized in several genes involved in the immune response and energetic pathways (Sauvage, 2007). In this context, the mapping of SNPs, of 16 new EST-SSR based markers and of... |
| Tipo: Text |
Palavras-chave: Mortality; Genetic; Crassostrea gigas; Pacific oyster; Mapping QTL. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-3504.pdf |
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| Lallias, Delphine; Stockdale, Ruth; Lapegue, Sylvie; Boudry, Pierre; Beaumont, Andy. |
| The European flat oyster Ostrea edulis and the blue mussel Mytilus edulis are both very valuable commercial species across Europe. Despite their economical importance, only a few microsatellite markers have so far been developed in those species. The aim of this study was to develop new microsatellites for O. edulis and M. edulis that could be used for population genetics studies, genetic variability assessment of stocks, parentage analysis or genetic and QTL mapping studies. For Ostrea edulis, an enriched library was made by ecogenics GmbH (Zurich, Switzerland) from size selected genomic DNA ligated into SAULA/SAULB-linker and enriched by magnetic bead selection with biotin-labelled (GT)13 and (CT)13 oligonucleotide repeats (Gautschi et al. 2000a,b). Of... |
| Tipo: Text |
Palavras-chave: M13 tail; Blue mussel; European flat oyster; Microsatellite. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4536.pdf |
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| Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois. |
| Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes. |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf |
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| Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Camara, Mark D.; Lapegue, Sylvie. |
| Marine species with high fecundity and high early mortality may also have high variance in reproductive success among individuals due to stochastic factors, making successful reproduction a "sweepstakes." In some cases, the impact is sufficient to reduce the effective number of breeders in wild populations. We tested two predictions of the sweepstakes reproductive success hypothesis in a French Atlantic population of the European flat oyster, Ostrea edulis, by evaluating (1) whether individuals belonging to temporally discrete recruitment cohorts within a single reproductive season displayed reduced genetic variation relative to the entire adult population, and (2) whether these temporal cohorts of recruits were genetically differentiated from each other.... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7399.pdf |
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| Huvet, Arnaud; Lapegue, Sylvie; Magoulas, A; Boudry, Pierre. |
| The respective status of the Portuguese oyster, Crassostrea angulata, and the Pacific oyster, Crassostrea gigas, has long been a matter of controversy. Morphological and physiological similarities, homogeneity of allozyme allelic frequencies between populations of the two taxa and the demonstration of hybridization lead most authors to suggest that they should be regrouped within the same species. The risk of introgression and the present expansion of C. gigas aquaculture in Europe raises the question of the need for preservation of C. angulata in Europe, as only a few populations remain. We studied European and Asian populations of C. gigas and C. angulata using microsatellite and mitochondrial DNA markers to estimate their genetic diversity and... |
| Tipo: Text |
Palavras-chave: Phylogeography; Population genetics; Microsatellites; Crassostrea gigas; Crassostrea angulata. |
| Ano: 2000 |
URL: https://archimer.ifremer.fr/doc/2000/publication-707.pdf |
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| Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean. |
| We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops. |
| Tipo: Text |
Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf |
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| Huvet, Arnaud; Fabioux, Caroline; Mccombie, Helen; Lapegue, Sylvie; Boudry, Pierre. |
| The marine environment is of special interest for studying hybridization between closely related taxa because of the high dispersal potential of planktonic larvae, such as those of most bivalve species. The oysters Crassostrea angulata and C. gigas are known to be very close genetically and entirely inter-fertile under controlled conditions. However, hybridization in the wild had not been investigated, mainly due to the lack of nuclear diagnostic markers. In the present paper, we first estimated genetic differentiation between these 2 closely related taxa using 8 microsatellite markers. Interestingly, 5 markers displayed significant differences of allele size between taxa. The subsequent sequencing of alleles of one of these microsatellites showed several... |
| Tipo: Text |
Palavras-chave: Allele size; Microsatellites; Genetic differentiation; Hybridization; Crassostrea gigas; Crassostrea angulata. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-3355.pdf |
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| Pouvreau, Stephane; Fleury, Elodie; Petton, Sebastien; Corporeau, Charlotte; Lapegue, Sylvie. |
| Occupant une place de premier ordre dans les paysages côtiers français, l’ostréiculture est le premier contributeur de la filière aquacole française avec une production estimée à 85 000 tonnes ces dernières années. Véritable espèce ingénieur, l’huître creuse, installée en France depuis plus de 50 ans, joue aussi un rôle essentiel sur le plan écologique dans les écosystèmes qui l’abritent. Pourtant, depuis plusieurs années, cette filière subie une baisse de production et les bancs naturels, garants de la diversité génétique, sont mis en danger. Ces phénomènes sont la conséquence directe de l’épizootie causée par OsHV-1 (sous sa forme µVar) affectant les naissains et des mortalités d’adultes associées à la bactérie Vibrio aestuarianus. Ces épisodes de... |
| Tipo: Text |
Palavras-chave: Huître creuse; Physiologie; Génétique; Ecologie; Réseau National. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00449/56030/57556.pdf |
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| Sauvage, Christopher; Pepin, Jean-francois; Lapegue, Sylvie; Boudry, Pierre; Renault, Tristan. |
| Ostreid herpes virus 1 (OsHV-1) infections, notably reported in Europe and the USA, are closely associated with significant mortalities of the Pacific oyster, Crassostrea gigas, especially during its early stages of life. In summer 2006, we monitored mortality by strict daily verification of three full-sib families of oysters reared under common conditions. We quantified OsHV-1 using real-time PCR in dead and living individuals during and after a mortality event. Mortality events were severe and brief, but significantly different between tested families (cumulative mortality ranging from 1.2 to 49%). Real-time PCR assays revealed different viral DNA loads in dead individuals from different families (P < 0.001). Moreover, the mean level of infection... |
| Tipo: Text |
Palavras-chave: Viral DNA quantification; Real time PCR; OsHV 1; Mortality; Crassostrea gigas. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6265.pdf |
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| Lapegue, Sylvie; Boudry, Pierre. |
| Oysters are among the most familiar of all marine invertebrate taxa. However our knowledge of oyster phylogeny and systematics is fragmentary. This is principally due to the plastic growth patterns of these animals, which result in a wide range of overlapping, ecophenotypic variants that greatly reduce the value of analysis based on shell morphology. Besides that, many intentional or accidental anthropogenic transfers have emphasised this situation. In several cases of misclassification or misidentification of oysters, DNA molecular data, have provided valuable new insights on the tracing of introductions or more generally on the phylogeographic relationships between oyster species. We will illustrate this issue with recent case studies from our lab on... |
| Tipo: Text |
Palavras-chave: Genetic; Ostreola stentina; Crassostrea honkongensis; Crassostrea gigas; Crassostrea angulata; Oysters; Population genetics. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3475.pdf |
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| Lapegue, Sylvie; Boudry, Pierre; Goulletquer, Philippe. |
| Originating from the north eastern Asia, Crassostrea gigas is endemic to Japan, but has been introduced and translocated, mainly for aquaculture purpose, into several countries, almost worldwide (1). In North America, the species can be found from Southeast Alaska to Baja California, while in European waters the species is cultured from Norway to Portugal as well as in Mediterranean Sea (Fig.1) (2). Biological characteristics make it suitable for a wide range of environmental conditions, although it is usually found in coastal and estuarine areas within its natural range. Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (3). |
| Tipo: Text |
Palavras-chave: Genetics; Ecology; Crassostrea gigas; Biology; Oysters. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3322.pdf |
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| Registros recuperados: 140 | |
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